Device

Part:BBa_K3024015:Design

Designed by: Tobias Willi   Group: iGEM19_Stockholm   (2019-10-06)


Switch plasmid for lytic lysogenic growth of P2 phage


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2336
    Illegal BamHI site found at 1282
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 997
    Illegal AgeI site found at 1109
    Illegal AgeI site found at 1421
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2295
    Illegal BsaI.rc site found at 3002


Design Notes

designed to transkribe in opposite directions


Source

Assembled from 2 parts of the registry

References